Abstract
Background
The aim of our study was to investigate the influence of the Trp64Arg polymorphism in the beta 3 adrenoreceptor gene on adipocytokine response, insulin
resistance, and weight loss secondary to lifestyle modification (Mediterranean hypocaloric
diet and exercise) in obese patients.
Methods
A population of 65 obese (BMI >30) non-diabetic outpatients was analyzed in a prospective way. Before and after 3 months
of a lifestyle modification program, indirect calorimetry, bioimpedance, blood pressure,
serial assessment of nutritional intake for 3 days via written food records, and biochemical
analysis were performed. The lifestyle modification program consisted of a hypocaloric
diet (1520 kcal, 52% carbohydrates, 25% lipids, and 23% proteins). The exercise program
consisted of an aerobic exercise at least three times a week (60 min each). The statistical
analysis was performed for Trp64/Arg64 and Arg64/Arg64 combined as a mutant group and for type Trp64/Trp64 as a wild group.
Results
The mean age of the study participants was 45.8±16.8 years and the mean BMI was 34.4±4.6 kg. There were 18 males (27.7%) and 47 females (72.3%). Fifty-five patients (84.6%;
15 males/40 females) had the Trp64/Trp64 genotype (wild group) and ten patients (15.4%; 3 males/7 females) had Trp64/Arg64 (mutant group). In the wild group, BMI, weight, fat mass, systolic blood pressure
and waist circumference decreased. In this group, resting metabolic rate (RMR), RMR
corrected by fat free mass, and VO2 increased. In the mutant group, BMI, weight, fat mass, and waist circumference decreased.
A significant increase was detected in RMR and VO2 as well. No differences were detected between baseline values in the two groups.
CRP levels were higher in the mutant group than in the wild variants. No differences
were detected in other parameters. Only leptin levels decreased significantly in both
the wild group (10.1%; p<0.05) and the mutant group (13.6; p<0.05).
Conclusion
This study showed that the beneficial effect of mild weight reduction by a low caloric
diet and exercise program is the greatest in subjects with a normal homozygote beta
3 adrenoreceptor gene.
Keywords
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Article info
Publication history
Published online: September 10, 2007
Accepted:
April 19,
2007
Received in revised form:
March 8,
2007
Received:
November 21,
2006
Identification
Copyright
© 2007 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
