Original article| Volume 10, ISSUE 4, P214-217, December 1999

Electron spin resonance spectroscopy for examination of human ischemic heart disease


      Background: Reports in the literature consistently expose the role of free radical reactions in human cardiovascular diseases. Studies in vivo are hindered by the short life span of free radicals and by the toxicity of trapping substances. Electron spin resonance (ESR) spectroscopy has thus been suggested as an indirect method for studying free radical reactions in ischemic heart disease. Methods: ESR spectra were obtained from blood samples of 25 healthy subjects (control group) and 43 patients with ischemic heart disease. Results: ESR spectra in the control and patients groups reflected a slightly asymmetric singlet state. Spectra in the patient group had a conspicuously weaker amplitude and were deformed. Conclusions: The amplitude and shape of ESR signals from blood of patients with ischemic heart disease may be of diagnostic and prognostic value.


      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to European Journal of Internal Medicine
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


        • Gonet B
        Free-radical method for l-ascorbic acid determination in tissues of experimental animals.
        Curr Top Biophys. 1994; 18: 202-204
        • Gonet B
        Effect of a magnetic field on ascorbate system in mice.
        Physiol Chem Phys Med NMR. 1991; 23: 239-245
        • Wójcicki J
        • Samochowiec L
        • Gonet B
        • et al.
        Effect of buckwheat extract on free radical generation in rabbits administrated high-fat diet.
        Phytother Res. 1995; 9: 323-326
      1. Pincemail J. Free radicals and antioxidants in human diseases. In: Favier AE, et al., editors. Analysis of free radicals in biological systems. Basel: Birkhäuser Verlag, 1995:83–98.

        • McCord J
        Oxygen-derived free radicals in post ischemic tissue injury.
        N Engl J Med. 1985; 312: 159-163
        • Thompson JA
        • Hess ML
        The oxygen free radical system: a fundamental mechanism in the production of myocardial necrosis.
        Prog Cardiovasc Dis. 1986; 28: 449-462
        • Pietri S
        • Culcasi M
        • Cozzone PJ
        Real-time continuous-flow spin trapping of hydroxyl radical in the ischemic and post-ischemic myocardium.
        Eur J Biochem. 1989; 186: 163-173
        • Coghlan JG
        • Fittler WD
        • Isley CD
        • et al.
        Reperfusion of infarcted tissue and free radicals.
        Lancet. 1991; 338: 1145
        • Wang X
        • Liu J
        • Yokoi I
        • et al.
        Direct detection of circulating free radicals in the rat using electron spin resonance spectrometry.
        Free Radical Biol Med. 1992; 12: 121-126
      2. Buettner GR, Jurkiewicz BA. Ascorbate radical: a valuable marker of oxidative stress. In: Favier AE et al., editors. Analysis of free radicals in biological systems. Basel: Birkhäuser Verlag, 1995:145–164.

        • Gey KF
        • Stähelin HB
        • Eichholzer M
        Poor plasma status of carotene and vitamin C is associated with higher mortality from ischemic heart disease and stroke.
        Basel Prospective Study: Clin Invest. 1993; 71: 3-6
        • Mosca L
        • Rubenfire M
        • Mandel C
        • et al.
        Antioxidant nutrient supplementation reduces the susceptibility of low density lipoprotein to oxidation in patients with coronary artery disease.
        J Am Coll Cardiol. 1997; 30: 392-399
        • Brown CJ
        Lack of vitamin C linked to heart attacks.
        Can Med Assoc J. 1997; 156: 1373
        • Van de Vijver LPL
        • Kardinaal AFM
        • Grobbee DE
        • et al.
        Lipoprotein oxidation, antioxidants and cardiovascular risk: epidemiologic evidence.
        Prostaglandins, Leukotrienes Essent Fatty Acids. 1997; 57: 479-487
        • Güler K
        • Palanduz S
        • Ademoglu E
        • Salmayenli N
        • et al.
        Total antioxidant status, lipid parameters, lipid peroxidation and glutathione levels in patients with acute myocardial infarction.
        Med Sci Res. 1998; 26: 105-106